Product Profile

Product Name	MYD88 Antibody
Antibody Type	Primary Antibodies
Product descriptio	MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-κB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-κB activation induced by IL-1, but not by NF-κB. The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues. Hide
Immunogen	MYD88 antibody was raised against amino acids 233 - 248 of MYD88 (Human).

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	IHCWB
	MYD88 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.:
Species Reactivity	HumanMouse
Concentration	1 mg/ml
Purification	Protein G

Target Information

Gene Symbol	MYD88
Alternative Names	MYD88 MYD88D
Tissue Specificity	Because of high sequence homology, this is expected to detect multiple isoforms of MyD88 protein.

Database Links

Entrez Gene	4615
Protein Accession	Q99836

Application

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Application

Immunohistochemistry staining of MYD88 in skin tissue using MYD88 Antibody.

Application Notes

MYD88 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.:

Additional Information

Form	Liquid
Storage Instruction	MYD88 antibody can be stored short term 4 ?C. For long term storage aliquot and store at -20 ?C. As with all antibodies avoid freeze/thaw cycles.
Storage Buffer	PBS, 0.02% sodium azide.