Product Profile

Product Name	Anti-MITF antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MITF
Gene Synonyms	MI WS2 CMM8 WS2A COMMAD bHLHe32 Hide
Gene Full Name	melanocyte inducing transcription factor
Gene Summary	The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017] Hide
Molecular Weight(MW)	59 kDa
Source	Rabbit
Cellular Localizatio	Nucleus

Application

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WB

Western blot analysis using MITF antibody against A431 (1), COS-7 (2), Hep G2 (3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded pancreatic cancer tissues using MITF antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using MITF antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.