Product Profile

Product Name	Anti-MCL1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MCL1
Gene Synonyms	TM EAT MCL1L MCL1S Mcl-1 BCL2L3 MCL1-ES bcl2-L-3 mcl1/EAT Hide
Gene Full Name	MCL1 apoptosis regulator, BCL2 family member
Gene Summary	This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010] Hide
Molecular Weight(MW)	37 kDa
Source	Rabbit
Cellular Localizatio	Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm

Application

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WB

Western blot analysis using MCL1 antibody against Hep G2 (1), A431 (2), NIH 3T3 (3), HT 1080 (4), COS-7 (5) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCL1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of HEK293 cells using MCL1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.