Product Profile

Product Name	Anti-EIF5A antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	EIF5A
Gene Synonyms	FABAS EIF-5A EIF5A1 eIF-4D eIF5AI Hide
Gene Full Name	eukaryotic translation initiation factor 5A
Gene Summary	Enables U6 snRNA binding activity and protein N-terminus binding activity. Involved in several processes, including cellular response to virus; positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator; and tumor necrosis factor-mediated signaling pathway. Located in annulate lamellae; cytoplasm; and nucleus. Part of nuclear pore. [provided by Alliance of Genome Resources, Apr 2022] Hide
Molecular Weight(MW)	17 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

Application

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WB

Western blot analysis using EIF5A antibody against B16 (1), Hela (2), Hep G2 (3), KATOⅢ (4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of MCF-7 cells using EIF5A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.