Product Profile

Product Name	Anti-Ku70 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	XRCC6
Gene Synonyms	ML8 KU70 TLAA CTC75 CTCBF G22P1 Hide
Gene Full Name	X-ray repair cross complementing 6
Gene Summary	The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	70 KDa
Source	Rabbit
Cellular Localizatio	Nucleus. Chromosome.

Application

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WB

Western blot analysis using Ku70 antibody against A549 (1), MCF-7 (2), HepG2 (3), COS-7 (4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Ku70 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of T24 cells using Ku70 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.