Product Profile

Product Name	Anti-SETDB1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SETDB1
Gene Synonyms	SETB1 ESET KG1T KMT1E KIAA0067 H3-K9-HMTase4 SETDB1 Hide
Gene Full Name	SET domain bifurcated histone lysine methyltransferase 1
Gene Summary	This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011] Hide
Molecular Weight(MW)	180 kDa（Observed:120 kDa）
Source	Rabbit
Cellular Localizatio	Chromosome, Cytoplasm, Nucleus

Application

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WB

Western blot analysis using SETDB1 antibody against 3T3-L1 (1), C2C12 (2), NIH3T3 (3), P19 (4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SETDB1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of HepG2 cells using SETDB1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.