Product Profile

Product Name	Anti-Prohibitin antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	PHB
Gene Synonyms	PHB PHB1 HEL-215 HEL-S-54e Hide
Gene Full Name	prohibitin
Gene Summary	This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Hide
Molecular Weight(MW)	30 KDa
Source	Rabbit
Cellular Localizatio	Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Application

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WB

Western blot analysis using Prohibitin antibody against K562 (1), PANC-1 (2),HepG2 (3),A431 (4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Prohibitin antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using Prohibitin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.