Product Profile

Product Name	Anti-Hsc70 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HSPA8
Gene Synonyms	HSPA8 HSP7C LAP1 HSC54 HSC70 HSC71 HSP71 HSP73 LAP-1 NIP71 HEL-33 HSPA10 HEL-S-72p Hide
Gene Full Name	heat shock protein family A (Hsp70) member 8
Gene Summary	This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Hide
Molecular Weight(MW)	71 kDa
Source	Rabbit
Cellular Localizatio	Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome

Application

·        

WB

Western blot analysis using Hsc70 antibody against A431(1), HepG2(2) cell lysate.

·       

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using Hsc70 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of Hela cells using Hsc70 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.