Product Profile

Product Name	Anti-YB1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	YBX1
Gene Synonyms	YB1 BP-8 CSDB DBPB YB-1 CBF-A CSDA2 EFI-A NSEP1 NSEP-1 MDR-NF1 Hide
Gene Full Name	Y-box binding protein 1
Gene Summary	This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	36 kDa
Cellular Localizatio	Cytoplasm, Nucleus, Secreted

Application

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WB

Western blot analysis using YB1 antibody against THP-1(1), HA431(2, Raji(3), HepG2(4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using YB1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of SK-OV-3 cells using YB1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.