Product Profile

Product Name	Anti-MDM2 antibody
Antibody Type	Primary Antibodies
Immunogen	recombinant protein

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MDM2
Gene Synonyms	HDMX LSKB hdm2 ACTFS Hide
Gene Full Name	MDM2 proto-oncogene
Gene Summary	This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] Hide
Molecular Weight(MW)	55 kDa（Observed: 80 kDa）
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using MDM2 antibody against HEK293(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MDM2 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using MDM2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.