Product Profile

Product Name	Anti-PTPN11 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	PTPN11
Gene Synonyms	CFC NS1 JMML SHP2 BPTP3 PTP2C METCDS PTP-1D SH-PTP2 SH-PTP3 Hide
Gene Full Name	protein tyrosine phosphatase non-receptor type 11
Gene Summary	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016] Hide
Molecular Weight(MW)	68 kDa
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using PTPN11 antibody against HepG2(1),THP-1(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PTPN11 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using PTPN11 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.