Product Profile

Product Name	Anti-ISG15 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	ISG15
Gene Synonyms	G1P2 IP17 UCRP IFI15 IMD38 hUCRP Hide
Gene Full Name	ISG15 ubiquitin like modifier
Gene Summary	The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] Hide
Molecular Weight(MW)	18 kDa
Cellular Localizatio	Cytoplasm, Secreted

Application

        

WB

Western blot analysis using ISG15 antibody against PANC-1(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissues using ISG15 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using ISG15 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.