Product Profile

Product Name	Anti-BRAF antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Protein

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	BRAF
Gene Synonyms	NS7 B-raf BRAF1 RAFB1 B-RAF1 BRAF-1 Hide
Gene Full Name	B-Raf proto-oncogene, serine/threonine kinase
Gene Summary	This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. Hide
Molecular Weight(MW)	84 kDa
Cellular Localizatio	Cell membrane, Cytoplasm, Membrane, Nucleus

Application

·        

WB

Western blot analysis using BRAF antibody against C2C12(1), T24(2) cell lysate.

·        

ICC/IF

Immunofluorescence analysis of Hela cells using BRAF antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.