Product Profile

Product Name	Anti-P100 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	TPX2
Gene Synonyms	DIL2 p100 DIL-2 HCTP4 FLS353 HCA519 REPP86 C20orf1 C20orf2 GD:C20orf1 More
Gene Full Name	TPX2 microtubule nucleation factorprovided
Gene Summary	Enables importin-alpha family protein binding activity and protein kinase binding activity. Involved in activation of protein kinase activity; microtubule cytoskeleton organization; and negative regulation of microtubule depolymerization. Located in intercellular bridge; mitotic spindle; and nucleoplasm. Colocalizes with spindle pole. More
Molecular Weight(MW)	86 kDa
Cellular Localizatio	Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Application

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WB

Western blot analysis using P100 antibody against Hela(1),Jurkat(2),HEK293(3),MCF-7(4)cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using P100 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of PC-3 cells using P100 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.