Product Profile

Product Name	CREB-2 Antibody (H-290) AC
Antibody Type	Primary Antibodies
Modification Notes	Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA (1,2). Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3 (3–6). These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains (5,6). Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family (7)

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFELISAGSChIP
	recommended for detection of CREB-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and porcine:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Alternative Names	epitope corresponding to amino acids 1-290 of CREB-2 of human origin
Tissue Specificity	epitope corresponding to amino acids 1-290 of CREB-2 of human origin

Database Links

Entrez Gene

468

Application

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Application

Western blot analysis of CREB-2 expression in K-562 whole cell lysate.

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Application

Western blot analysis of CREB-2 expression in non-transfected: (A) and human CREB-2 transfected: (B) 293T whole cell lysates.

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Application

Western blot analysis of CREB-2 expression in non-transfected 293T: (A), human CREB-2 transfected 293T: (B) and HeLa (C) whole cell lysates.

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Application

Western blot analysis of CREB-2 expression in HeLa nuclear extract (A) and A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.

Application Notes

recommended for detection of CREB-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and porcine:

Additional Information

Form	Liquid
Storage Instruction	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.