Product Profile

Product Name	claudin-5 Antibody (H-52)
Antibody Type	Primary Antibodies
Modification Notes	The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human Claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrone, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFELISA
	recommended for detection of claudin-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Alternative Names	epitope corresponding to amino acids 167-218 mapping at the C-terminus of claudin-5 of human origin
Tissue Specificity	epitope corresponding to amino acids 167-218 mapping at the C-terminus of claudin-5 of human origin

Database Links

Entrez Gene

7122

Application

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Application

Western blot analysis of claudin-5 expression in non-transfected: (A) and human claudin-5 transfected: (B) 293T whole cell lysates.

Application Notes

recommended for detection of claudin-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine:

Additional Information

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.