Product Profile

Product Name	COX2 antibody
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal [4H12] to COX2 / Cyclooxygenase 2COX2 is a mitogen-inducible form of Cyclooxygenase, which is expressed in response to various inflammatory stimuli. COX2 staining increases in the more differentiated, suprabasal keratinocytes of normal skin. COX2 is expressed in epithelial and interstitial cells of adenomatous polyps and Colon adenocarcinomas. Hide
Immunogen	Prokaryotic recombinant protein corresponding to amino acids 30 to 163 of the N-terminus of the human COX-2 molecule.

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	4H12
Host Species	Mouse
Tested Applications	IHC-P
	IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user.: Hide
Species Reactivity	Human
Concentration	1 mg/ml

Target Information

Gene Synonyms	PHS-2 COX-2 hCox2 hCox-2 PGHS-2 PGG/HS PGHS2 5743 600262 COX2 PTGS2 P35354 PHS2 PHS 2 COX 2 hCox 2 PGHS 2 Hide
Target	COX2
Cellular Localization	Microsomal membrane

Application

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Application

IHC-P analysis of colon carcinoma using COX2 antibody [4H12].

Positive Control	Colon Cancer section
Application Notes	IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user.: Hide

Additional Information

Form	Liquid
Storage Instruction	Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer	Tissue Culture Supernatant containing 0.05% sodium azide