羧化红色荧光纳米金刚石：Carboxylated Red Fluorescent Nanodiamond

Biofunctionalized fluorescent nanodiamonds are infinitely photostable labels for fluorescent and multiphoton microscopy applicable to cellular tracking and long-term observation of biological processes in the same fields of view in live cell-imaging and fixed cells. Protein, antibody, biologically active organic molecules, and custom functionalizations are possible. We provide nanodiamonds with nitrogen-vacancy (NV) centers with red-to-near-infrared fluorescence, NVN centers with green fluorescence, and novel particles available only at Adamas with a combination of NV and NVN centers within a single particle with visual yellow fluorescence. Fluorescence from NV centers can be magnetically modulated providing a means to eliminate the fluorescent background by image subtraction and to significantly enhance the signal-to-noise ratio. This property can be very useful in future biosensing applications, for example in clinical diagnostics such as lateral flow assays (LFA). Relevant applications also include super-resolution microscopy, multiphoton microscopy, time-gated microscopy and correlative microscopy.