推荐：Beta-Amyloid (1-42)

推荐：Beta-Amyloid (1-42)

Beta amyloid proteins 聚集指南（以下方案仅供参考，请根据您的具体实验需求进行调整）:


1. 将Aβ肽(1-42)粉末溶于冷的六氟-2-丙醇（HFIP）。肽溶液在室温下孵化至少1小时，以建立单体化和随机化的结构。(Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.)



2. 蒸发掉HFIP，将得到的多肽以薄膜形式保存在-20或-80℃。(The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.)



3. 将得到的薄膜以5 mM的浓度溶解于无水DMSO中，然后用涡旋方式稀释到适当的浓度和缓冲液（不含血清和酚红的培养基）中。(The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.)



4. 接下来，该溶液在4-8℃下老化48h。然后在4-8 ℃下以14000g离心10分钟；可溶性低聚物在上清液中。将上清液稀释10-200倍用于后续实验。(Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.)



以上方法因后续不同应用而异(Methods vary depends on the downstream applications.)。



注意：老化后由于在溶液中聚集形态不稳定，建议现配现用。