推荐：异硫氰酸胍 for molecular biology, ≥99%

别名:硫氰酸亚脲, 硫氰酸胍

线性分子式:NH2C(=NH)NH2 · HSCN

CAS号:593-84-0

分子量:118.16

Beilstein:3563461

EC 号:209-812-1

MDL编号:MFCD00013027

PubChem化学物质编号:24895367

NACRES:NA.31

生物来源
synthetic (organic)

质量水平
200

等级
for molecular biology

检测方案
≥99%

形式
powder or crystals

储存条件
(Tightly closed. Dry. )

technique(s)
RNA extraction: suitable

颜色
colorless to white,


mp
120-122 °C (lit.)

溶解性
water: 100 mg/mL, clear, colorless

密度
1.29 g/cm3 at 20 °C (68 °F)

痕量阳离子
Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

application(s)
agriculture

异质活性
DNase, RNase, none detected

SMILES string
SC#N.NC(N)=N

InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N

一般描述
Guanidine thiocyanate, or guanidinium rhodanide, is a versatile chemical compound widely used in molecular biology and biochemical research. Acting as a chaotropic agent, it disrupts cell and organelle membranes, facilitating cell lysis and organelle isolation. Notably, it serves as a potent protein denaturant, surpassing guanidine hydrochloride in unfolding proteins. This property is valuable for purifying nucleic acids from crude cell lysates, effectively separating nucleic acids from denatured proteins.

In nucleic acid purification, guanidine thiocyanate is effective in inactivating DNases and RNases, preventing nucleic acid degradation and ensuring favorable yields. Its dual denaturing and enzyme-inhibiting properties make it crucial for DNA and RNA studies. Beyond nucleic acid isolation, guanidine thiocyanate is used for cell lysis and virus particle inactivation during RNA and DNA extractions. It not only disrupts membranes but also denatures RNase and DNase enzymes, protecting extracted nucleic acids from enzymatic degradation. The versatility of guanidine thiocyanate extends to molecular biology techniques like PCR, Northern blotting, and Southern blotting. It serves as a vital reagent in these methods, contributing to the successful analysis and amplification of nucleic acids.
应用
胍硫氰酸盐已用于：
RNA分离之前裂解大肠腺癌细胞系的[1]
作为三重线粒体DNA (mtDNA) qPCR反应体系中的PCR抑制剂[2]
用于从脑脊液（CSF）中提取DNA的qPCR分析[3]

离液剂和 变性剂;溶解细胞。
生化/生理作用
胍硫氰酸盐是一种变性剂，常用于RNA分离。它可用作全血样品的储存缓冲液。[4]胍硫氰酸盐可灭活核酸酶，是储存和冷冻粪便样本进行DNA研究的理想选择。[5]它可与苯酚?氯仿联用提取RNA。[6]
特点和优势
Highly versatile surfactant for your cell biology and biochemical research
Suitable for RNA extraction in sensitive molecular biology applications
Free from Dnase and Rnase
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

免责声明
试剂盒仅供研究使用，不得用于临床实验或生物体实验，否则所产生的一切后果，由实验者承担， 本公司概不负责。
严格按照说明书操作，不同批号不可交换使用，实验者违反说明书操作，后果由实验者承担。



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