推荐：革兰氏结晶紫 溶液 for microscopy

别名:Basic violet 3; gentian violet; hexamethyl pararosaniline; methyl violet 10B

NACRES:MA.02

属性
等级
for microscopy

质量水平
200

产品线
BioChemika

形式
liquid

保质期
limited shelf life, expiry date on the label

technique(s)
microbe id | staining: suitable

密度
0.981 g/mL at 20 °C

εmax
33.8 at 585-595 nm in water

抗生素 谱
Gram-negative bacteria
Gram-positive bacteria

application(s)
diagnostic assay manufacturing
hematology
histology

储存温度
room temp

说明
一般描述
Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.[1][2]
应用
Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues.
It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.
生化/生理作用
The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionized dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.[2]
原理
Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms complexes with Gram’s iodine that are entrapped and retain the purple color even after decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner and does not retain crystal violet.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元