推荐：Bactident™ Kovac′s Indole reagent@2024已更新

别名:4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

属性
质量水平
100

形式
liquid (ready-to-use)

保质期
210 days

特点
ready-to-use

组成
(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

包装
pkg of (1 x 30 ml, Plastic bottle)

technique(s)
microbe id | specific enzyme detection: suitable

pH值（酸碱度）
<1 (20 °C in H2O)

转变温度
flash point 36 °C

密度
0.92 g/cm3 at 20 °C

application(s)
environmental
food and beverages

相容性
Bactident™ E. coli 1.13303

储存温度
2-8°C

适用性
Enterobacter spp.
Escherichia coli

说明
一般描述
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
应用
Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.
联系
Replaces: 1.11350.0007
分析说明
Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

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SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      1F≥10                1000元