推荐：O'Meara 试剂@2024已更新

别名:使用 O'Meara 试剂的 Voges-Proskauer 试验

NACRES:NA.85

属性
产品线
BioChemika

质量水平
100

保质期
limited shelf life, expiry date on the label

组成
creatine, 0.3 g
dist. water, 100 mL
potassium hydroxide, 40 g

包装
pkg of 100 mL

储存条件
(Tightly closed. Dry.)

technique(s)
microbe id | metabolite detection: suitable

application(s)
environmental
food and beverages
microbiology

适用性
Enterobacter spp.
Klebsiella spp.
Neisseria spp.
Pseudomonas spp.

说明
一般描述
O′Meara′s Reagent is used in the Voges-Proskauer (VP) test for the detection of acetoin production by bacterial strains. VP test is helpful in identifying members of the family Enterobacteriaceae based on their ability to produce acetylmethylcarbinol (AMC = acetonin) from glucose. Acetoin is an intermediate which is oxidized by oxygen under alkaline conditions (KOH) to 2,3-Butanediol. With creatinine (break down product from creatine) and the enhancement of α-naphtol a red color is formed (Meisenheimer complex)
应用
The O′Meara reagent is a component of the Voges-Proskauer (VP) test, which is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce acetylmethylcarbinol (AMC) from glucose. The VP test is often used as a tool to identify and authenticate bacterial strains in the food, beverage, and pharmaceutical industries.
分析说明
To perform the VP test, a bacterial sample is inoculated into a nutrient broth and incubated. The O′Meara reagent, which contains a mixture of creatinine and potassium hydroxide (KOH), is then added to the culture. Acetoin produced in the medium by bacterial culture is oxidized under alkaline conditions in the presence of air to form diacetyl which reacts with creatine to give a pinkish-red compound.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元