推广：O157 Millichrome™ plus Agar

suitable for microbiology

别名:E. coli O157 chromogenic agar

UNSPSC代码:41161501

NACRES:NA.74

属性
Agency
EP
JP
USP

质量水平
200

灭菌适用性
dry heat compatible

形式
dry powder

保质期
limited shelf life, expiry date on the label

组成
Agar, 15.0 g/L
Chromogenic Mix, 1.2 g/L
Chromogenic mix, 1.2 g/L
Peptone and yeast
extract, 13.0 g/L
Peptone & Yeast Extract, 13.0 g/L

制造商/商品名称
MilliChrome™

技术
microbiological culture: suitable

最终pH
6.9±0.2 (25 °C)

溶解性
deionized water: 29.2 g/L

应用
food and beverages
microbiology

运输
ambient

储存温度
15-25°C

适用性
selective for bacteria: Escherichia coli O157

说明
一般描述
O157 Millichrome™ plus Agar is a selective media for the isolation and detection of E. coli O157. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli O157. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. For optimal growth, peptone and yeast extract provide nitrogenous nutrients and essential growth factors. Sodium chloride is crucial for maintaining osmotic balance. The chromogenic mix contains substrates that change color when characteristic enzymes cleave them, allowing for easy differentiation.To create a more selective and specific medium, it is recommended to include a potassium tellurite supplement. If there is expected to be a high load of Proteus spp., cefixime can be added. Similarly, a high load of Pseudomonas and/or Aeromonas can be inhibited by including cefsulodin. Agar serves as the solidifying agent.
应用
O157 Millichrome™ plus Agar Base is a selective chromogenic culture medium intended for use in the qualitative direct detection, differentiation and presumptive identification of pathogenic biotypes of E. coli O157 from various samples.
制备说明
Step 1 (Preparation)
Disperse slowly 29.2 g of powder base in 1 L of purified water.
Stir until agar is well thickened.
Heat and bring to boil (100 °C) while swirling or stirring regularly.
DO NOT HEAT TO MORE THAN 100 °C. DO NOT AUTOCLAVE AT 121 °C.
Warning: If using an autoclave, do so without pressure.

Advice 1: For the 100 °C heating step, mixture may also be brought to a boil in a microwave oven: after initial boiling, remove from oven, stir gently, then return to oven for short repeated bursts of heating until complete fusion of the agar grains has taken place (large bubbles replacing foam).
Advice 2: if a more selective, and more specific, medium is needed, add a solution of Potassium Tellurite to obtain a final concentration of 2.5 mg/L at 45-50 °C.
Advice 3: in case of product samples containing a high load of Proteus, Cefixime can be added at 0.025 mg/L at 45-50 °C.
Advice 4 : in case of product samples containing a high load of Pseudomonas and/or Aeromonas, Cefsulodin can be added at 5 mg/L at 45-50 °C.

Step 2 (Pouring plates)
Cool in a water bath at 45-50 °C, swirling or stirring gently.
Pour into sterile Petri dishes.
Let it solidify and dry.
储存及稳定性
Store prepared media below 8°C, protected from direct light (max. 1 month).Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.
分析说明
The E. coli O157 is detected by a characteristic mauve color after only 24 h of incubation at an optimal growth temperature of 37°C, while most other E. coli form blue color colonies after incubation at the same temperature
法律信息
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

IF<5，赠送价值 500元礼品卡
5 ≤IF≤10，赠送价值 1000元礼品卡
IF≥10，赠送价值 1000元礼品卡+任意 1 款 ValukineTM 试剂盒


DUMABIO ELISA试剂盒文献征集奖励活动

为感谢各位老师对笃玛品牌的支持与厚爱，凡购买DUMABIO ELISA试剂盒，在SCI期刊杂志发表文中注明ELISA试剂盒采购上海笃玛生物科技有限公司（Shanghai DuMa Biotechnology Co., Ltd.）

Shanghai DuMa Biotechnology Co., Ltd.

发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
SCI期刊杂志      3.0≤1F＜6.0          400元
SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元