Product Profile

Product Name	Anti-HMGB1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HMGB1
Gene Synonyms	HMG1 HMG3 HMG-1 SBP-1 Hide
Gene Full Name	high mobility group box 1
Gene Summary	This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	25 kDa
Source	Rabbit
Cellular Localizatio	Cell membrane, Chromosome, Cytoplasm, Endosome, Membrane, Nucleus, Secreted

Application

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WB

Western blot analysis using HMGB1 antibody against Hep G2 (1), T24 (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using HMGB1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of SK-OV-3 cells using HMGB1 antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.