Product Profile

Product Name	Anti-NF-kBp65 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	RELA
Gene Synonyms	p65 CMCU NFKB3 RELA TF65 Hide
Gene Full Name	RELA proto-oncogene, NF-kB subunit
Gene Summary	NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Hide
Molecular Weight(MW)	60 kDa
Source	Rabbit
Cellular Localizatio	Nucleus. Cytoplasm.

Application

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WB

Western blot analysis using NF-kBp65 antibody against Hep G2 (1), COS-7 (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NF-kBp65 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of SK-OV-3 cells using NF-kBp65 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.