Product Profile

Product Name	Anti-CCNB1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CCNB1
Gene Synonyms	Cyclin B1 CCNB
Gene Full Name	cyclin B1
Gene Summary	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017] Hide
Molecular Weight(MW)	48 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Cytoskeleton, Nucleus

Application

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WB

Western blot analysis using CCNB1 antibody against PC-3 (1) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CCNB1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using CCNB1 antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.