Product Profile

Product Name	Anti-EGR1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	EGR1
Gene Synonyms	TIS8 AT225 G0S30 NGFI-A ZNF225 KROX-24 ZIF-268 Hide
Gene Full Name	early growth response 1
Gene Summary	The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014] Hide
Molecular Weight(MW)	58 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using EGR1 antibody against HepG2 (1), Jurkat (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using EGR1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of 3T3-L1 cells using EGR1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.