Product Profile

Product Name	Anti-Calnexin antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CANX
Gene Synonyms	CNX P90 IP90
Gene Full Name	calnexin
Gene Summary	This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018] More
Molecular Weight(MW)	68 kDa（Observed:100 kDa）
Source	Rabbit
Cellular Localizatio	Endoplasmic reticulum membrane. Melanosome

Application

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WB

Western blot analysis using Calnexin antibody against A549 (1), K562 (2), COS-7 (3), Hela (4), HEK293 (5) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Calnexin antibody with DAB staining.

·       

ICC/IF

Immunofluorescence analysis of COS-7 cells using Calnexin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.