Product Profile

Product Name	Anti-PLK1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	PLK1
Gene Synonyms	PLK STPK13
Gene Full Name	polo like kinase 1
Gene Summary	The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	68 KDa
Source	Rabbit
Cellular Localizatio	Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Application

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WB

Western blot analysis using PLK1 antibody against HepG2 (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using IPLK1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using PLK1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.