Product Profile

Product Name	Anti-PFKFB2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	PFKFB2
Gene Synonyms	PFK-2/FBPase-2
Gene Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Gene Summary	The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	58 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm.Nucleus

Application

·        

WB

Western blot analysis using PFKFB2 antibody against Jurkat (1), A549 (2), SK-N-SH (3), NIH 3T3 (4) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PFKFB2 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of Hela cells using PFKFB2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.