Product Profile

Product Name	Anti-DNMT3A antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	DNMT3A
Gene Synonyms	TBRS HESJAS DNMT3A2 M.HsaIIIA Hide
Gene Full Name	DNA methyltransferase 3 alpha
Gene Summary	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] Hide
Molecular Weight(MW)	102 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using DNMT3A antibody against HepG2 (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using DNMT3A antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Huvec cells using DNMT3A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.