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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | 电询 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Anti-WT1 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
WT1
Gene Synonyms
GUD
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Gene Full Name
WT1 transcription factor
Gene Summary
This gene encodes a transcription factor
that contains four zinc-finger motifs at the C-terminus and a
proline/glutamine-rich DNA-binding domain at the N-terminus. It has an
essential role in the normal development of the urogenital system, and it is
mutated in a small subset of patients with Wilms tumor. This gene exhibits
complex tissue-specific and polymorphic imprinting pattern, with biallelic,
and monoallelic expression from the maternal and paternal alleles in
different tissues. Multiple transcript variants have been described. In
several variants, there is evidence for the use of a non-AUG (CUG)
translation initiation codon upstream of, and in-frame with the first AUG.
Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA
editing in human and rat, and that this process is tissue-restricted and
developmentally regulated. [provided by RefSeq, Mar 2015]
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Molecular
Weight(MW)
49 KDa
Source
Rabbit
Cellular
Localizatio
Cytoplasm, Nucleus
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
AWT1
WAGR
WT33
NPHS4
WIT-2
·
WB
Western blot analysis using WT1 antibody against MCF-1 (1) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using WT1 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of T24 cells using WT1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |