Product Profile

Product Name	Anti-HK2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HK2
Gene Synonyms	HKII HXK2 DKFZp686M1669 HK2 Hide
Gene Full Name	hexokinase 2
Gene Summary	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009] Hide
Molecular Weight(MW)	102 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Application

·       

WB

Western blot analysis using HK2 antibody against HepG2 (1) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HK2 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of A549 cells using HK2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.