Product Profile

Product Name	Anti-ILK antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	ILK
Gene Synonyms	P59 ILK-1 ILK-2 p59ILK HEL-S-28 Hide
Gene Full Name	integrin linked kinase
Gene Summary	This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] Hide
Molecular Weight(MW)	51 KDa
Source	Rabbit
Cellular Localizatio	Cell junction > focal adhesion. Cell membrane.

Application

        

WB

Western blot analysis using ILK antibody against A549 (1), HepG2 (2), A431(3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ILK antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of HepG2 cells using ILK antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.