Product Profile

Product Name	Anti-PCSK9 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	PCSK9
Gene Synonyms	FH3 PC9 FHCL3 NARC1 LDLCQ1 NARC-1 HCHOLA3 Hide
Gene Full Name	proprotein convertase subtilisin/kexin type 9
Gene Summary	This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Hide
Molecular Weight(MW)	74 KDa
Source	Rabbit
Cellular Localizatio	Secreted, Plasma Membrane,endoplasmic reticulum,lysosome, cytoplasm.

Application

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WB

Western blot analysis using PCSK9 antibody against A431(1), THp-1(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCSK9 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of NIH3T3 cells using PCSK9 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.