Product Profile

Product Name	Anti-LDHA antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	LDHA
Gene Synonyms	LDHM GSD11 PIG19 HEL-S-133P Hide
Gene Full Name	lactate dehydrogenase A
Gene Summary	The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] Hide
Molecular Weight(MW)	37 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm

Application

·        

WB

Western blot analysis using LDHA antibody against HepG2 (1), PANC-1 (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using LDHA antibody with DAB staining.

        

ICC/IF

Immunofluorescence analysis of NIH3T3 cells using LDHA antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.