Product Profile

Product Name	Anti-Rb antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	RB1
Gene Synonyms	RB1；RB pRb OSRC pp110 p105-Rb PPP1R130 p110-RB1 Hide
Gene Full Name	RB transcriptional corepressor 1
Gene Summary	The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	106 KDa
Source	Rabbit
Cellular Localizatio	Nucleus

Application

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WB

Western blot analysis using Rb antibody against COS7 (1), A549 (2), PC-3 (3), A431 (4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using RB antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using RB antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.