Product Profile

Product Name	Anti-NOX2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CYBB
Gene Synonyms	CY24B CYBB CGD NOX2 IMD34 AMCBX2 GP91-1 GP91PHOX p91-PHOX GP91-PHOX Hide
Gene Full Name	cytochrome b-245 beta chain
Gene Summary	Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	65 KDa
Source	Rabbit
Cellular Localizatio	Membrane

Application

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WB

Western blot analysis using NOX2 antibody against HUVEC (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissues using NOX2 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using NOX2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.