Product Profile

Product Name	Anti-AIF antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AIFM1
Gene Synonyms	AIFM1； AIF AUNX1 CMT2D CMTX4 COWCK DFNX5 NADMR NAMSD PDCD8 COXPD6 SEMDHL Hide
Gene Full Name	apoptosis inducing factor mitochondria associated 1
Gene Summary	This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015] Hide
Molecular Weight(MW)	67 KDa
Source	Rabbit
Cellular Localizatio	Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus.

Application

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WB

Western blot analysis using AIF antibody against A549 (1), Raji (2), HUVEC (3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using AIF antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using AIF antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.