Product Profile

Product Name	Anti-β-Actin antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Proteins

Key Feature

Clonality	Monoclonal
Isotype	IgG
Host Species	Mouse
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanRabbit
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	ACTB
Gene Synonyms	Act Actx beta-a beta-actin E430023M04Rik Hide
Gene Full Name	actin, beta
Gene Summary	This gene encodes a member of the actin family of proteins. Actins are highly conserved proteins that are among the most abundant proteins in eukaryotic cells and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the transcribed mRNA are regulated through the binding of multiple factors to its 3' UTR sequence. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous pseudogenes of this gene have been identified in the mouse genome. [provided by RefSeq, Sep 2015] Hide
Molecular Weight(MW)	42 kDa
Source	Mouse
Cellular Localizatio	Cytoplasm, Cytoskeleton, Nucleus

Application

·        

WB

Western blot analysis using Beta-Actin antibody against A431 (1), A549 (2), COS7 (3), Hep G2 (4) and HEK293 (5) cell lysate.

·        

ICC/IF

Immunofluorescence analysis of A431 cells using Beta-Actin antibody (green). Blue: DAPI fluorescent DNA dye.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.