Product Profile

Product Name	Anti-DDX4 antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Proteins

Key Feature

Clonality	Monoclonal
Isotype	IgG
Host Species	Mouse
Tested Application	ELISAIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000
Species Reactivity	HumanRabbit
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	DDX4
Gene Synonyms	VASA
Gene Full Name	DEAD-box helicase 4
Gene Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Hide
Molecular Weight(MW)	28 kDa, 47 kDa, 76 kDa
Source	Mouse
Cellular Localizatio	Cytoplasm

Application

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WB

Western blot analysis using DDX4 antibody against Hep G2 (1), HEK 293 (2) and MCF-7 (3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using DDX4 antibody with DAB staining.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.