Product Profile

Product Name	Anti-MAP2K2 antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Proteins

Key Feature

Clonality	Monoclonal
Isotype	IgG
Host Species	Mouse
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanRabbit
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MAP2K2
Gene Synonyms	CFC4 MEK2 MKK2 MAPKK2 PRKMK2 Hide
Gene Full Name	mitogen-activated protein kinase kinase 2
Gene Summary	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	44 kDa
Source	Mouse
Cellular Localizatio	Cytoplasm, Membrane

Application

        

WB

Western blot analysis using MAP2K2 antibody against HEK293 (1), HT1080 (2), U251 (3), Hep G2 (4) and C2C12 (5) cell lysate.

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ICC/IF

Immunofluorescence analysis of PC-3 cells using MAP2K2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.