Product Profile

Product Name	Anti-AKT2 antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Proteins

Key Feature

Clonality	Monoclonal
Isotype	IgG
Host Species	Mouse
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanRabbit
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AKT2
Gene Synonyms	PKBB PRKBB HIHGHH PKBBETA RAC-BETA More
Gene Full Name	AKT serine/threonine kinase 2
Gene Summary	This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019] More
Molecular Weight(MW)	55 kDa
Source	Mouse
Cellular Localizatio	Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus

Application

·

WB

Western blot analysis using AKT2 antibody against HEK 293 (1), C2C12 (2), Hep G2 (3) and HT1080 (4) cell lysate.

·

IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using AKT2 antibody with DAB staining.

·

ICC/IF

Immunofluorescence analysis of A431cells using AKT2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.

什么样的检验要求？要出示什么吗？
怎样算是处理过？还是就只是离心保存？
答：离心保存，检验要求，就是标本标号，和特殊要求
问：测好多指标 血清不分开可以么？
答：要分开，分好后，分别标上序列号，便于实验好区分。