Product Profile

Product Name	Anti-SIRT1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SIRT1
Gene Synonyms	SIR2 SIR2L1 SIR2alpha
Gene Full Name	sirtuin 1
Gene Summary	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] Hide
Molecular Weight(MW)	82 kDa, 120 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Mitochondrion, Nucleus

Application

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WB

Western blot analysis using SIRT1 antibody against COS-7 (1), A549 (2), NIH3T3 (3), HepG2 (4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using SIRT1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of U251 cells using SIRT1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.