Product Profile

Product Name	Anti-OCT-4 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	POU5F1
Gene Synonyms	OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 Hide
Gene Full Name	POU class 5 homeobox 1
Gene Summary	This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] Hide
Molecular Weight(MW)	39 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using OCT-4 antibody against PANC-1(1), HepG2(2),HEK293(3),A431(3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using OCT-4 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using OCT-4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.