Product Profile

Product Name	Anti-Smad3 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SMAD3
Gene Synonyms	LDS3 LDS1C MADH3 JV15-2 HSPC193 HsT17436 Hide
Gene Full Name	SMAD family member 3
Gene Summary	The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. It also functions as a tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, Nov 2019] Hide
Molecular Weight(MW)	48 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm. Nucleus.

Application

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WB

Western blot analysis using Smad3 antibody against PANC-1(1), A431(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Smad3 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using Smad3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.