Product Profile

Product Name	Anti-AP2M1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AP2M1
Gene Synonyms	mu2 AP50 MRD60 CLAPM1 Hide
Gene Full Name	adaptor related protein complex 2 subunit mu 1
Gene Summary	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015] Hide
Molecular Weight(MW)	50 kDa
Cellular Localizatio	Cell membrane, Coated pit, Membrane

Application

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WB

Western blot analysis using AP2M1 antibody against Hela(1), NIH/3T3(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using AP2M1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of SK-OV-3 cells using AP2M1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.