Product Profile

Product Name	Anti-SGCA antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SGCA
Gene Synonyms	ADL DAG2 50DAG DMDA2 LGMD2D LGMDR3 SCARMD1 adhalin Hide
Gene Full Name	sarcoglycan alpha
Gene Summary	This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] Hide
Molecular Weight(MW)	43 kDa
Cellular Localizatio	Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Application

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WB

Western blot analysis using SGCA antibody against PANC-1(1), MCF-7(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using SGCA antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of SK-OV-3 cells using SGCA antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.