Product Profile

Product Name	Anti-SERCA2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	ATP2A2
Gene Synonyms	DD DAR ATP2B SERCA2 Hide
Gene Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
Gene Summary	This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019] Hide
Molecular Weight(MW)	115 kDa
Cellular Localizatio	Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

Application

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WB

Western blot analysis using SERCA2 antibody against A431(1),MCF-1(2),Hela(4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded renal carcinoma tissues using SERCA2 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using SERCA2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.